International Tomography Center
Siberian Branch of Russian Academy of Sciences

Equipment

Equipment for mass-spectrometry and sample preparation.

  • LC/MS Hybrid ultrahigh resolution electrospray quadrupole time-of-flight (UHR ESI-q-TOF) mass spectrometer MaXis 4G (Bruker Daltonik GmbH) with Ultra-HPLC system Dionex UltiMate3000RS;
  • LC/MS ESI Ion Trap Esquire 6000 (Bruker Daltonik GmbH) with HPLC system Agilent 1200 cap/nano;
  • MALDI-TOF/TOF mass spectrometer Ultraflex III TOF/TOF (Bruker Daltonik GmbH);
  • MALDI-TOF mass spectrometer Autoflex II (Bruker Daltonik GmbH);
  • HPLC system Agilent 1200 with autosampler and UV detection (diode array);
  • Set of electrophoretic equipment Protean (Bio-Rad Laboratories) for 1D- and 2D-electrophoresis;
  • Imaging system VersaDoc 4000MP (Bio-Rad Laboratories) for electrophoretic gel imaging and subsequent post-processing;
  • Automatized sample preparation system ClinProt robot (Bruker Daltonik GmbH);
  • MALDI imaging system (Bruker Daltonik GmbH) for analysis of spatial distribution of protein biomarkers, metabolites and drugs in thin tissue slices for biological and clinical research;
  • Sample preparation system ImagePrep (Bruker Daltonik GmbH) for MALDI imaging system;
  • Microbial identification system MALDI BioTyper (Bruker Daltonik GmbH);
  • Multimode microplate reader Infinite F200 (Tecan).
  • Microtome-cryostat CryoStar NX70 for tissue slicing (Thermo Scientific);

Photophysical / photochemical equipment.

  • Spectrophotometer Agilent 8453;
  • Spectrofluorimeter system FLSP920 (Edinburgh Instruments) for steady-state and time-resolved fluorescence measurements. Excitation sources: LED 270, 300 and 330 nm (FWHM = 800 ps); Laser diode 375 nm (FWHM = 80 ps). Additional options: magnetic field in 0-700 gauss region; solid sample holders; low temperature measurements (77 K).
  • Laser Flash Photolysis setup. Excitation sources: 266, 355 и 532 nm (Nd:YAG3+ laser) and 308 nm (excimer XeCl laser); detection on selected wavelengths in 240-700 nm region; time resolution = 10 ns. Additional options: magnetic field in 0-3000 gauss region; temperature variation in 200-360 К region;
  • Steady-state photolysis setup. Excitation source: high-pressure mercury lamp 1000 W, a set of optical filters in 250-1000 nm region.

Other equipment.
  •  Pack of standard physical and chemical laboratory equipment (thermostats, shakers, centrifuges, pH-meters and others);
  • Personal computers and software packages for post-processing of raw data and analysis of obtained data and images.